Chromosome organization during cell division and effects on nuclear shape in interphase

Kelly Xu, Vermont, USA 16-18

Many cancerous cells often have misshapen or lobed nuclei. However, the mechanisms of this are currently unknown. Here, the goal was to determine if chromosome organization during mitosis had an impact on nuclear shape. To do this, kinesin Kif18A was knocked out of the cell, which essentially disrupted chromosome organization. Then, using imaging and computer analysis, the roundness (solidity) of the nuclei was measured. It was evident that with the knockouts, an increased percentage of abnormally shaped nuclei were seen.

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